TABLE 1.

Nucleotide sequences and positions of primers used in this study

PrimeraSequencebPositionGenBank accession no.
GFP15′-TAGCAGATCTAAAATGAGTAAAGGAGAAGAAC-3′286-307 U62636
GFP25′-TCGGAGATCTGTTGGAATTCATTATTTGTAGAG-3′994-1026 U62636
SB25′-GTGAAGCACCAGTTTCTGAACC-3′4184-4205 AF012877
SR55′-GGTCAGATCTAGCCAGAATTAAAAGTTAGTG-3′4141-4171 AF012877
SR85′-AAGTTCGGATCCTGCTTTTGGTGGTGC-3′5184-5210 AF012877
SR125′-GTGTATCAGCTGTCGGAAC-3′4513-4531 AF012877
SR145′-TGTCGGATCCACATCAGTGGTTGCATGTAAT-3′4523-4553 AF012877
SR165′-CTTTTTGATCTGAATAGTTTCCCGCATCAC-3′5068-5097 AF012877
SR175′-TGTCGGAACAAGATCTGTGGTTGCATGTAAT-3′4523-4553 AF012877
SR235′-CTTGTGAATCACCACCAGATG-3′5380-5400 AF012877
SR245′-GCAAGTCAAGCGGGAC-3′4069-4084 AF012877
TET85′-GGAGAAATCCCTGCTCGGTG-3′1957-1976 X56353
CJ65′-CAATTACCAACCATGTTAGC-3′3576-3595 U89875
CJ85′-GGTTAGTAATGCTGATCGC-3′2328-2346 U89875
U5′-GTAAAACGACGGCCAGT-3′379-395 L09137
R5′-GGAAACAGCTATGACCATG-3′461-479 L09137
  • a Primers GFPn, SB2 and SRn, Tet8, CJn, and U and R refer to GFP, the spiralin gene of S. citri, the tetM gene of Tn916, the scm1 gene of S. citri, and the universal and reverse primers of pUC19, respectively.

  • b Restriction sites used for cloning are underlined.