TABLE 3.

Phylogenetic affiliations and frequency of isolates and clone libraries

Phylogenetic groupingArcticAntarcticNearest phylogenetic relativea% Identical 16S rRNAb
No. of isolatesNo. of clonesNo. of isolatesNo. of clones
ARKXIII/2ARKXV/1ARKXVI/2ANTV/2ANTXI/4_13ANTXI/4_14
Bacteria115861068710395
α-Proteobacteria
    Roseobacter134Antarctic quartz stone sublithic isolate QSSC9-5 (AF170750)93.0-97.8
21Adriatic Sea isolate AS-26 (AJ391187)97.2-99.7
564Roseobacter sp. strain Shippagan (AF100168)98.1-98.9
2Roseobacter litoralis (X78312)98.0
314North Atlantic continental slope isolate EI-2 (AF254103)96.9-98.0
297581Octadecabacter arcticus (U73725)97.0-99.0
5Ligurian Sea isolate SRF3 (AJ002565)98.1
7Mediterranean Sea clone MED-6 (AF025549)97.0-98.3
1Nankai Trough clone NKB7 (AB013259)95.1
    Sphingomonas2Antarctic seawater isolate SW54 (U85838)96.5
1Erythrobacter longus (M59062)97.8
β-Proteobacteria
1Soil ultramicrobacterium strain ND5 (AB008506)97.8
γ-Proteobacteria
    Shewanella18Shewanella frigidimarina ACAM584 (U85902)99.3-99.7
    Psychromonas33Psychromonas antarctica (U14697)97.7-99.5
    Colwellia591Colwellia psychroerythraea IC064 (U85842)98.2-98.9
152163740Colwellia sp. strain ANT5-9 (AF19350)97.4-99.6
9Colwellia rossensis ACAM608 (U14589)99.3-99.7
44578Colwellia-like sp. strain IC169 (AF001376)98.0-98.2
    Glaciecola519232515Gas vacuolate sea ice bacterium strain 214.6 (GVstr214.6 [U73724])97.6-98.7
10224Glaciecola punicea ACAM 611T (U85853)97.0-98.3
454Glaciecola pallidula ACAM 615T (U85854)98.6-98.7
    Pseudoalteromonas2Pseudoalteromonas antarctica (X98336)99.7-100
    Methylophaga1Methylophaga marina (X95459)94.5
    Psychrobacter412313Psychrobacter glacincola IC084 (U85876)98.1-99.7
1Psychrobacter marincola (PMA309941)99.6
3Psychrobacter sp. strain St1 (AF260715)98.0-99.5
    Acinetobacter1Acinetobacter johnsonii (X89775)99.5
1Acinetobacter lwoffii (U10875)98.8
    Pseudomonas4Pseudomonas sp. strain ACAM213 (U85868)98.3-100
2Arctic seawater bacterium R7366 (UBA293826)99.4
    Marinobacter81311Marinobacter sp. strain IC022 (U85863)97.1-99.0
18Marinobacter sp. strain DS40M8 (AF199440)95.0-99.7
1Marinobacter hydrocarboniclasticus (AB021372)91.2
    Oceanospirillum2112Oleispira antarctica (AJ426420)98.4-99.3
11Marinomonas protea (AJ238597)96.0-99.4
    Halomonas2Halomonas variabilis strain SW04 (U85873)98.6-99.8
1Gamma Proteobacterium MBIC3958 (AB020600)95.1
1Antarctic shelf clone MERTZ_2CM_20 (AF424059)97.0
    Terredinibacter2Arctic sea ice clone ARCTIC_ICE-12 (AF277547)98.1
    NOR5c1North Sea clone KTc1119 (AF235120)95.1
CFPolaribacter filamentus (U73726)98.9-99.5
    Polaribacter21Arctic Ocean clone Arctic 96B-11 (AF354621)93.7
138Polaribacter franzmannii (U14586)97.9-98.1
1427Polaribacter irgensii (M61002)99.1-99.3
    Cellulophaga33Mesocosm diatom bloom isolate 5.3.10 (AF367849)98.0-98.3
    Flavobacterium3Flavobacterium xanthum IC001 (U85889)98.7-99.1
    Salegentibacter7Salegentibacter salegens (M92279)97.9-99.1
    Psychroflexus22Psychroflexus torquis ACAM623 (U85881)98.1-98.7
16North Atlantic abyssal isolate AIII4 (AF254114)92.9-96.2
    Psychroserpens6Psychroserpens burtonensis ACAM188 (U62913)94.0-95.8
    Cytophaga25Arctic Ocean clone Arctic96AD-4 (AF354619)96.8-98.1
1Japan Trench clone JTB132 (AB015260)98.8
1Antarctic sea ice clone SIC.42370 (AF277483)93.8
Green nonsulfur bacteria1Arctic Ocean clone Arctic 95A (AF355054)87.3
Actinobacteria2Nesterenkonia halobia (X80847)97.9
11Soil ultramicrobacterium str. 121 (AB008511)97.8-98.0
1Brachybacterium faecium (X91032)91.8
1Microbacterium sp. VKM Ac-2047 (AB042082)99.4
3Freshwater clone HTG6 (AF418963)91.7-92.0
Total 16S rRNA phylotypes331519201311
Shannon index ± variance1.291 ± 0.0021.065 ± 0.0021.091 ± 0.0011.127 ± 0.0020.844 ± 0.0020.812 ± 0.001
Coverage (%)53.373.684.681.8
  • a In ARB database.

  • b Estimated in ARB.

  • c Reference 24.