TABLE 4.

Frequency of bacterial phylotypes in Arctic and Antarctic pack ice observed by FISH in comparison to clone and isolate libraries

Probe target groupProbeResults for samples collected in thea:
ArcticAntarctic
FISHbClone librarycIsolatesdFISHbClone librarycIsolatesd
ARKXV/1ARKXVI/2ARKXV/1ARKXVI/2ARKXIII/2ANTXI/4_13ANTXI/4_14ANTXI/4_13ANTXI/4_14ANTV/2
BacteriaEUB33894 ± 595 ± 5N/AN/AN/A93 ± 696 ± 4N/AN/AN/A
α-ProteobacteriaALF96828 ± 633 ± 79134226 ± 524 ± 47424
    Roseobacter cladeROSEO53627 ± 828 ± 59133916 ± 57 ± 27424
        Octadecabacter spp.ODB102119 ± 523 ± 58525<1<1109
    Roseobacter sp. strain Shippagan groupRSHP995<1<10013 ± 32 ± 16155
    Sphingomonas spp.SPH120<1<10034 ± 3<1000
β-ProteobacteriaBET42a7 ± 65 ± 41002 ± 22 ± 3000
γ-ProteobacteriaGAM42a49 ± 551 ± 971783034 ± 1140 ± 9888065
    Alteromonas-ColwelliaALT141330 ± 733 ± 92220419 ± 422 ± 4424617
    Pseudoalteromonas-ColwelliaPSA18432 ± 1137 ± 14532916 ± 519 ± 5272241
        Glaciecola sp. strain GVstr214.6GVstr2144 ± 5<101843 ± 4<1251828
        Glaciecola puniceaGPU6225 ± 53 ± 401003 ± 23 ± 3241
    Marinobacter sp. strain IC022 groupMB-IC02223 ± 1322 ± 81417<1<1001
    Psychrobacter spp.PSYB4764 ± 39 ± 31275 ± 17 ± 3133
    Shewanella frigidimarinaSF8253 ± 27 ± 4051<1<1000
    Psychromonas spp.NOR2-1453<11 ± 1030<1<1003
    Vibrio spp.GV<1<1000<13 ± 3000
    Oceanospirillum spp.OCE2323 ± 51 ± 121111 ± 15 ± 4200
    Pseudomonas spp.PS56a<1<1105<1<1000
CF groupCF319a22 ± 428 ± 61862331 ± 534 ± 48179
    Polaribacter spp.PB2232 ± 1<100328 ± 635 ± 75176
ActinobacteriaHGC69a<1<1034<1<1001
PlanctomycetalesPLA46<1<1000<1<1000
ArchaeaARCH915<1<1000<1<1000
  • a Data present results obtained during the indicated research cruises; for more information, please refer to the text.

  • b Values represent the mean percent total cells observed with DAPI staining ± standard deviation.

  • c Values represent the percent total 16S rRNA gene clones. N/A, not applicable.

  • d Values represent the percent total isolates. N/A, not applicable.