Methane production by the three instars of P. ephippiata larvae and by isolated midguts and hindguts (third instar) incubated separately in Ringer's solution under air

Larva or gut sectionCH4 production rate (μmol g−1 h−1)an
    First instar0.13 ± 0.044
    Second instar0.12 ± 0.034
    Third instar0.36 ± 0.116
Isolated gut sections
    Hindgut0.030 ± 0.00714
  • a All values are based on the fresh weight of the whole animal and are means ± standard errors for n different larvae.

  • b ND, not detectable (<0.1 nmol g−1 h−1).