TABLE 1.

Yeast strains used in this study

StrainGenotype or strain (plasmids)Background and/or description
MB329-17Cα trp1 ura3-52 put1-54 PRO1S288C and ∑1278b
FH515α trp1 ura3-52 put1-54 mutated PRO1MB329-17C, l-Proline-accumulated mutant
INVSc1a his31 leu2 trp1-289 ura3-52S288C, His Leu Trp Ura
INVDput1a his31 leu2 trp1-289 ura3-52 put1::CgHIS3INVSc1, put1 disruptant, Leu Trp Ura
INV-WTINVSc1 (pAD4, pTV3, pUV2, pHV1)INVSc1, wild type
INVDput1-WTINVDput1 (pAD4, pTV3, pUV2)INVSc1, put1 disruptant
INVDput1-W1INVDput1 (pAD-WTPRO1, pTV3, pUV2)High-copy PRO1
INVDput1-M1INVDput1 (pAD-D154NPRO1, pTV3, pUV2)High-copy mutated PRO1
INVDput1-W12INVDput1 (pAD-WTPRO1, pTV-PRO2, pUV2)High-copy PRO1, PRO2
INVDput1-M12INVDput1 (pAD-D154NPRO1, pTV-PRO2, pUV2)High-copy mutated PRO1, PRO2
INVDput1-W123INVDput1 (pAD-WTPRO1, pTV-PRO2, pUV-PRO3)High-copy PRO1, PRO2, PRO3
INVDput1-M123INVDput1 (pAD-D154NPRO1, pTV-PRO2, pUV-PRO3)High-copy mutated PRO1, PRO2, PRO3
INVDput1-W2INVDput1 (pAD4, pTV-PRO2, pUV2)High-copy PRO2
INVDput1-W3INVDput1 (pAD4, pTV3, pUV-PRO3)High-copy PRO3