TABLE 3.

Changes in the areas under lactic, formic, and acetic acid peaks and unknown peaks with RT = 15.3 (peak A) and RT = 17.0 (peak B) in single cultures of Pseudomonas sp. (CP), B. thermosphacta (CB), and S. putrefaciens (CS) and their mixed cultures MPB, MPS, MBS, and MPBS in gel cassette systems at 5°C

Cassette(s)Area under peaka at time (h):
04068114168238284
Lactic acid (RT = 10.4)
    CPNDNDNDNDNDNDNA
    CBNDNDND0.0240.0600.087NA
    CSNDNDNDNDNDNDNA
    MPBNDNDND0.0780.0880.0490.035
    MPSNDNDNDNDNDNDND
    MBSNDNDTR0.0760.1350.1820.122
    MPBSNDTRTR0.0740.1160.1910.083
Formic acid (RT = 11.3)
    CP0.1000.0820.0650.030NDNDNA
    CB0.1000.0800.0720.0580.0560.055NA
    CS0.1000.0900.1901.0170.8880.703NA
    MPB0.1000.0690.0700.0630.0500.040TR
    MPS0.1000.0650.0730.0400.0620.0580.051
    MBS0.1000.0200.0200.0590.0660.1220.027
    MPBS0.1000.0630.0660.1210.1500.2650.438
Acetic acid (RT = 12.4)
    CPNDNDNDNDNDNDNA
    CBNDTRTR0.0200.0400.049NA
    CSNDNDTRTR0.0450.060NA
    MPBNDTRTRTRTRTR0.020
    MPSNDTRTR0.0350.0430.0540.063
    MBSNDTRTR0.0320.0450.0600.060
    MPBSNDTRTR0.0300.0430.0490.056
Peak A (RT = 15.2)
    CP0.1010.1050.0500.040TRTRNA
    CB0.1010.1200.1150.0800.0600.030NA
    CS0.1010.1000.1130.4801.5671.850NA
    MPB0.1010.1210.0900.030TRTRTR
    MPS0.1010.1060.0900.1431.0511.4001.123
    MBS0.1010.1330.1290.1000.1700.2100.192
    MPBS0.1010.1340.0940.0910.1250.1710.994
Peak B (RT = 17)
    CPNDNDNDNDNDNDNA
    CBNDNDNDNDNDNDNA
    CSNDNDTR0.3020.5800.674NA
    MPBNDNDNDNDNDNDND
    MPSNDNDND0.2460.2110.2720.249
    MBSNDNDTR0.1800.2280.2710.182
    MPBSNDNDTR0.2440.2470.2270.315
  • a Each sample value is the mean of two samples taken from different experiments (coefficient of variation of the mean of samples taken from different experiments of <5%). Each sample was analyzed in duplicate (coefficient of variation of samples from the same experiment of <0.65%). ND, not detected; NA, not analyzed; TR, trace amounts.