TABLE 1.

Examination of benzoates as competitors of 2-CBa uptake by 2-CBa-grown cells of strain D1

Test competitoraMetabolism of competitorbUptake rate (%)c
NoneNA100
Benzoate+92.4 ± 11.6
Substituted benzoates
    ortho
        Fluoro-+30.1 ± 0.2*
        Chloro-+51.8 ± 3.3*
        Bromo-+83.3 ± 6.3
        Iodo-+99.2 ± 4.4
        Hydroxy-+69.8 ± 3.2*
        Methyl-+63.4 ± 12.4*
        Nitro-ND107.7 ± 2.7
    meta
        Chloro-29.0 ± 2.0*
        Bromo-ND53.3 ± 4.4*
        Hydroxy-ND108.2 ± 4.4
    para
        Chloro-110.1 ± 6.9
        Bromo-ND122.0 ± 15.1
        Hydroxy-ND115.9 ± 16.7
    ortho and meta
        2,3-Dichloro-+89.1 ± 14.8
        2,5-Dichloro-+107.0 ± 17.3
        2,3,5-Trichloro-+104.6 ± 2.0
  • a Competitor and 2-[14C]CBa added in equal molar amounts (10μ M).

  • b Indication of whether the competitor molecule was previously determined (13, 14, 29) to be metabolized by 2-CBa-grown cells: +, competitor metabolized;− , competitor not metabolized; NA, not applicable; ND, not determined in previous experiments.

  • c 100% = 6.39± 1.39 nmol min−1 mg of protein−1. Each value is the mean of two replicate assays ± standard deviation of the mean. *, values significantly different (P < 0.01) from those of the control lacking a competitor.