TABLE 2.

PCR primers used in this work

PrimerSequenceSourcea
MON-FCGC AAT GTC TGG GTC GGC AAtftD, hadA
MON-RCAC GCG GCC GAT CTT CACtftD, hadA
HQ-FACC GCC ACC GGC CAC AAG TGhadC
HQ-RAGC GAT TCG CGC ACG CCG AAC ACA GhadC
MAR-FAAA TGA CGC CGA TCT ATG GmacA, tftE, tfdFI, tfdFII
MAR-RAGC ACA TGG CAG AGC TTG TGmacA, tftE, tfdFI, tfdFII
  • a tft genes from B. cepacia AC1100 (10); had genes from R. pickettii DTP0602 (28); macA from R. eutropha 335 (GenBank AF130250); tfd genes from R. eutropha JMP134(pJP4) plasmid. GenBank accession numbers: tftD and tftE, U83405; hadA and hadC, D86544; tfdFII, U16782; tfdFI, M35097.