TABLE 2.

Effect of metabolic inhibitors on 2-CBa uptake rates and cellular ATP levels measured with 2-CBa-grown cells of strain D1

InhibitoraConcn (mM)Uptake rate (%)bATP level (%)c
None0100100
Nigericin0.025122.8 ± 8.7108.0 ± 3.4
Valinomycin0.025102.7 ± 10.5115.4 ± 2.8
DCCD0.5106.3 ± 7.487.8 ± 13.5
VO4−31100.9 ± 27.2108.3 ± 27.9
AsO4−32096.3 ± 4.881.3 ± 15.6
AsO4−35056.4 ± 2.5*d36.1 ± 5.2*
AsO4−310046.9 ± 3.9*23 ± 8.1*
CCCP0.0222.3 ± 5.8*47.2 ± 2.1*
DNP11.9 ± 2.6*19.6 ± 2.1*
KCN102.1 ± 3.9*6.9 ± 0.1*
  • a Abbreviations: DCCD, N,N-dicyclohexylcarbodiimide; CCCP, carbonyl cyanide m-chlorophenylhydrazone; DNP, 2,4-dinitrophenol.

  • b 100% = 5.5 ± 0.6 nmol min−1 mg of protein−1.

  • c 100%= 4.8 ± 0.3 nmol mg of protein−1.

  • d *, values significantly different than the control lacking an inhibitor (P < 0.01).