TABLE 1.

Strains, plasmids, and primers used in this study

Strain, plasmid, or primerRelevant feature or sequenceSequenceSource or reference
Strains
    M. aeruginosa PCC 7806Microcystin-producing cyanobacteriumUNSW culture collection
    E. coli DH5αCloning hostUNSW culture collection
    E. coli BL-21(DE3)Expression hostUNSW culture collection
Plasmids
    pGEM-T EasyCloning vectorPromega
    pET-30aExpression vectorNovagen
Primers
    NFntcA degenerateCAGTGTTTTTGGGGTGYTTimothy Downing, University of Port Elizabeth
    NRntcAGTTTCAATCATCATTTCCGTTimothy Downing, University of Port Elizabeth
    NF/1ntcA pan-handleCGAGAGTAATTCTACCGGAGThis study
    NF/2ntcAGATCCCTCCGCAATAATCCCThis study
    NF/3ntcATTTCTACCTGCTCGATCGGThis study
    NF/4ntcAAGTGAACAGATAGCTGTGCThis study
    NF/5ntcACTGACCAAACGTGAACCCATThis study
    NF/6ntcACGAGTGTCACCTCTATTAAACACThis study
    NTCANDE1FntcA cloningTCCATATGGACTTATCATTAATACAAGATAAACThis study
    NTCAXHO1RntcA cloningTCCTCGAGAGTAAATTGTTGACTGAGAGCGThis study
    T7 termpET-30aGCTAGTTATTGCTCAGCGGTNovagen
    T7 prompET-30aTAATACGACTCACTATAGGGNovagen
    MPFpGEM-T EasyCCCAGTCACGACGTTGTAAAACGPromega
    MPRpGEM-T EasyAGCGGATAACAATTTCACACAGGPromega
    T7Pr1Adaptor primerCCCCTATCCACCCTTACACCTATC15
    M4FNtcA binding site in mcyA/D promoterCGAATTCTAATGATTTTTACTAATTTATTGGGThis study
    M4RNtcA binding site in mcyA/D promoterCCGCCGGCGAATTGTTCTGAGCCTCGACATThis study
    M5FNtcA binding site in mcyA/D promoterCGAATTCCCGTCGGGTTTCCTGTThis study
    M5RNtcA site in mcyA/D promoterCCGCCGGCGCATTGCTGTTCTAACTTTTTCCThis study
    M6FntcA promoterCGAATTCCAATAGCCGACCCCAGCGThis study
    M6RntcA promoterCCGCCGGCGTTTTTATTATCCAACGAGTGTCAThis study
    NtcArealFntcA reverse transcription-PCR short productCATTTCCGTTTGCAGAATCCThis study
    NtcArealRntcA reverse transcription-PCR short productTGTTTTTGGGGTGCTATCCTThis study
    RpoC1Frpoc1 reverse transcription-PCR short productCCTCAGCGAAGATCAATGGTThis study
    RpoC1Rrpoc1 reverse transcription-PCR short productCCGTTTTTGCCCCTTACTTTThis study
    McyBrealFmcyB reverse transcription-PCR short productCTGAGGGGATTACGGATTGAThis study
    McyBrealRmcyB reverse transcription-PCR short productACCATATAAGCGGGCAGTTGThis study