TABLE 3.

Positively regulated σB-dependent genes present in all four L. monocytogenes lineages

GeneaProductbLineage ILineage IILineage IIIALineage IIIB
Fold changecAdjusted P valuedFold changecAdjusted P valuedFold changecAdjusted P valuedFold changecAdjusted P valued
lmo0133Conserved hypothetical protein4.50.00035.1<0.00019<0.000114.5<0.0001
lmo0134Acetyltransferase, GNAT family6.90.00015.6<0.000114.3<0.00017.7<0.0001
lmo0169Similar to a glucose uptake protein5.50.00023.4<0.00015.1<0.00016.9<0.0001
lmo0170Conserved hypothetical protein3.60.000630.00085.6<0.00017.4<0.0001
lmo0210Similar to l-lactate dehydrogenase4.5<0.00012.6<0.00013.80.00014.60.0058
lmo0211Similar to B. subtilis general stress protein1.70.03351.50.00012.10.00212.4<0.0001
lmo0405Phosphate transporter family protein2.70.04101.70.01062.10.00532.10.0036
lmo0433Internalin A3.60.00043.1<0.00014.8<0.00017<0.0001
lmo0515Conserved hypothetical protein3.30.01163.40.00023.5<0.00015.4<0.0001
lmo0539Similar to tagatose-1,6-diphosphate aldolase14.4<0.00017.5<0.000119.5<0.000124.2<0.0001
lmo0555Similar to di-tripeptide transporter7.20.00024.1<0.00017.1<0.00019.3<0.0001
lmo0593Similar to transport proteins (formate)60.00325.7<0.000190.000318.2<0.0001
lmo0596Similar to unknown proteins14.20.000122.7<0.000133.8<0.000136<0.0001
lmo0602Weakly similar to transcription regulator2.60.00233.7<0.00012.80.00032.50.0121
lmo0610Similar to internalin proteins, putative peptidoglycan-bound protein (LPXTG motif)1.90.03803.7<0.00015.5<0.00018.2<0.0001
lmo0642Putative membrane protein3.40.002820.000220.00293.10.0005
lmo0655Similar to phosphoprotein phosphatases4.4<0.00012.9<0.00012.50.00143.10.0007
lmo0722Similar to pyruvate oxidase4.60.00025.4<0.00018.8<0.00013.80.0070
lmo0781Similar to mannose-specific PTS component IID10<0.000115.6<0.000118.2<0.000125.4<0.0001
lmo0782Similar to mannose-specific PTS component IIC12.9<0.000113.5<0.000120.3<0.000122<0.0001
lmo0783Similar to mannose-specific PTS component IIB6.4<0.000112<0.000114.8<0.000118<0.0001
lmo0784PTS system, IIAB component, authentic frameshift20.03415.7<0.00015.5<0.00016.4<0.0001
lmo0794Similar to B. subtilis YwnB protein6.10.000712.8<0.000110.3<0.000125.4<0.0001
lmo0796Conserved hypothetical protein1.90.00294<0.00018.3<0.000112.3<0.0001
lmo0880LysM domain protein7.4<0.00016.7<0.000116.4<0.00016.80.0006
lmo0896Indirect negative regulation of σB-dependent gene expression (serine phosphatase)1.50.02442.3<0.00011.70.01972.70.0017
lmo0911Unknown2.10.01522.1<0.00019.2<0.00011.80.0200
lmo0913Succinate semialdehyde dehydrogenase6.6<0.000113.4<0.000116<0.000122.3<0.0001
lmo0937Unknown6.4<0.000110.4<0.000116.2<0.000118.9<0.0001
lmo0953Unknown3.20.01566.5<0.000112<0.000116.3<0.0001
lmo0956Similar to N-acetylglucosamine-6-phosphate deacetylase (EC 3.5.1.25)2.60.00732.1<0.00014.4<0.00014.3<0.0001
lmo0957Glucosamine-6-phosphate isomerase2.30.00061.60.00273.10.00272.40.0441
lmo0994Unknown24.4<0.000114.1<0.000154.2<0.000179.2<0.0001
lmo1140Unknown40.00843.5<0.00014.90.00014.9<0.0001
lmo1241Conserved hypothetical protein2.70.00122.20.00024.2<0.00015<0.0001
lmo1295Similar to host factor 1 protein1.80.01203.4<0.00015.4<0.00018<0.0001
lmo1375Peptidase, M20/M25/M40 family2.40.01683<0.00012.40.00252.10.0381
lmo1425Similar to betaine-carnitine-choline ABC transporter (membrane protein)1.90.00623.4<0.00013.5<0.00014.7<0.0001
lmo1428Similar to glycine betaine-carnitine-choline ABC transporter (ATP-binding protein)2.20.00082.9<0.00012.70.00013.50.0004
lmo1433Pyridine nucleotide disulfide oxidoreductase family protein4.70.00064.2<0.000130.03783.70.0309
lmo1602Similar to unknown proteins4.2<0.00014.6<0.00011.70.04172.7<0.0001
lmo1605UDP-N-acetylmuramate-alanine ligase7.40.00012.2<0.00012.40.00472.8<0.0001
lmo1606FtsK/SpoIIIE family protein9.1<0.00015.6<0.00013.5<0.00014.1<0.0001
lmo1694Similar to CDP-abequose synthase8<0.00018<0.000126.2<0.00011.80.0162
lmo1799Putative peptidoglycan-bound protein (LPXTG motif)2.80.00171.70.00024.8<0.00014.7<0.0001
lmo2085Cell wall surface anchor family protein11<0.000112.2<0.000114.10.000716.80.0067
lmo2130Similar to unknown protein2.10.01122.6<0.00011.90.01042.30.0006
lmo2191Similar to unknown proteins2.20.00663<0.00012.3<0.00012.3<0.0001
lmo2269Unknown4.30.00965.7<0.00015.6<0.00016.2<0.0001
lmo2391Conserved hypothetical protein similar to B. subtilis YhfK protein6.2<0.00019.1<0.000121.6<0.000128.5<0.0001
lmo2434Glutamate decarboxylase gamma3.20.01932.7<0.000140.00973.8<0.0001
lmo2454Unknown3.60.00034.6<0.00016.6<0.00018.2<0.0001
lmo2463Similar to transport protein30.04263.9<0.00016.2<0.00014.30.0266
lmo2485PspC domain protein, truncated1.90.01134.4<0.00011.80.000620.0021
lmo2570Putative membrane protein5.5<0.00014.5<0.00017.3<0.00018.9<0.0001
lmo2571Similar to nicotinamidase4.4<0.00015.8<0.00017.3<0.00018.3<0.0001
lmo2572Similar to chain A, dihydrofolate reductase4.40.00021.60.00187.5<0.00013.5<0.0001
lmo2573Alcohol dehydrogenase, zinc dependent3.40.00024.6<0.00015.6<0.00017.5<0.0001
lmo2673Conserved hypothetical protein6.90.00099.7<0.000126<0.000137.2<0.0001
lmo2674Similar to ribose-5-phosphate epimerase9.8<0.00013.8<0.000110.1<0.000113.7<0.0001
lmo2724Similar to unknown proteins2.80.00302.9<0.00014.7<0.00014.9<0.0001
lmo2748Similar to B. subtilis stress protein YdaG11.6<0.000110<0.000115.3<0.00015.10.0063
lmo-f2365_0703Conserved hypothetical protein2.20.02773.3<0.00012.20.00382.40.0186
  • a Gene names are based on the L. monocytogenes EGDe locus.

  • b Common names of products are based on the EGDe annotation.

  • c Changes in the transcript level in the parent strain compared to the transcript level in the ΔsigB strain. A ≥1.5-fold change was considered significant.

  • d An adjusted P value of <0.05 was considered significant.