TABLE 1.

Generation times and maximum growth yields of L. lactis strains under nonstress and stress conditions

Condition(s)StrainGeneration time (min)aGeneration time relative to that of NZ-VectorMaximum OD600Maximum OD600 relative to that of NZ-Vector
30°C, without nisinNZ-Vector25.411.671
NZ-EDnaK23.30.921.671
NZ-LDnaK21.50.851.650.99
30°C, with nisinNZ-Vector60.311.491
NZ-EDnaK24.50.451.671.12
NZ-LDnaK46.00.761.650.99
40°C, without nisinNZ-Vector39.3311.21
NZ-EDnaK39.591.011.31.08
NZ-LDnaK37.000.941.31.08
40°C, with nisinNZ-VectorNDbc0.161
NZ-EDnaK47.10.986.24
NZ-LDnaK2180.764.85
3% NaCl, with nisinNZ-Vector13711.081
NZ-EDnaK65.00.471.371.28
5% Ethanol, with nisinNZ-Vector15011.071
NZ-EDnaK1160.781.201.13
0.5% Lactic acid, with nisinNZ-Vector20410.601
NZ-EDnaK78.50.390.861.44
  • a Generation time was determined in the exponential growth phase.

  • b ND, not detected (since the cells were not in exponential phase).

  • c —, uncalculated.