TABLE 2.

Physiology of the engineered S. cerevisiae strain IMZ132 and the empty-vector reference strain IME076 during anaerobic batch cultivation on synthetic medium (pH 5) with glucose-acetate mixturesa

Yeast strainRelevant genotypeGlycerol-3-phosphate dehydrogenase (μmol mg protein−1 min−1)Acetaldehyde dehydrogenase (acetylating) (μmol mg protein−1 min−1)Specific growth rate (h−1)Yield on glucose
Biomass (g mol−1)Glycerol (mol mol−1)Ethanol (mol mol−1)
Not corrected for evaporationCorrected for evaporation
IME076GPD1 GPD20.034 ± 0.003<0.0020.32 ± 0.0115.0 ± 0.10.14 ± 0.011.54 ± 0.021.61 ± 0.03
IMZ132gpd1Δ gpd2Δ + mhpF<0.0020.020 ± 0.0040.14 ± 0.0114.8 ± 1.6<0.0021.67 ± 0.041.82 ± 0.05
  • a Results are represented as average and mean deviations of data from two independent batch cultures. Glycerol yields on glucose were calculated using glycerol concentrations obtained by enzymatic determination.