TABLE 1.

Saccharomyces cerevisiae strains used in this study

StrainRelevant genotypeSource/reference
CEN.PK113-5DMATa ura3 GPD1 GPD2P. Kötter, Frankfurt
IME076MATa ura3 GPD1 GPD2 p426_GPD(URA3)This study
CEN.PK102-3AMATa ura3 leu2 GPD1 GPD2P. Kötter, Frankfurt
RWB0094MATa ura3 leu2 gpd1(−1,1133)::loxP-KanMX-loxP gpd2(−2,1281)::hphMX4BIRD Engineering, Rotterdam
IMZ008MATa ura3 leu2 gpd1(−1,1133)::loxP gpd2(−2,1281)::hphMX4 YEplac181(LEU2)This study
IMZ132MATa ura3 leu2 gpd1(−1,1133)::loxP gpd2(−2,1281)::hphMX4 YEplac181(LEU2) pUDE43(URA3 pTHD3::mhpF (E. coli)::CYC1t)This study
IMZ127MATa ura3 leu2 gpd1(−1,1133)::loxP gpd2Δ(−2,1281)::hphMX4 YEplac181(LEU2) p426_GPD(URA3)This study