TABLE 2.

Numbers of RNA copies and numbers of infectious particles per liter in raw sewage and treated effluent samples positive for astrovirus genogroup Ba

Sample location, dateNo. of RNA copies/liter of raw sewagebCC-RT-PCR U/liter of raw sewagecNo. of RNA copies/liter of treated effluentCC-RT-PCR U/liter of treated effluent
Balaks, Nov. 19983.8 × 1053.3 × 1039.9 × 1043.3 × 102
Balaks, Jan. 19998.7 × 1063.3 × 1031.1 × 1053.3 × 102
Balaks, May 19998.7 × 1053.3 × 1026.2 × 1053.3 × 101
El-Berka, Nov. 19981.1 × 1053.3 × 1031.1 × 103<3.3 × 100
El-Berka, July 19991.1 × 104<3.3 × 100NQ<3.3 × 100
El-Berka, Aug. 19991.1 × 104<3.3 × 100NQ<3.3 × 100
El-Berka, Sept. 19991.1 × 1053.3 × 102NQ<3.3 × 100
Zenin, Nov. 19989.9 × 1053.3 × 1031.1 × 103<3.3 × 100
Zenin, Dec. 19985.4 × 1063.3 × 105NQ<3.3 × 100
Zenin, Jan. 19996.2 × 1053.3 × 1021.1 × 104<3.3 × 100
Zenin, Feb. 19993.7 × 1053.3 × 1021.1 × 104<3.3 × 100
Zenin, Apr. 19991.1 × 1053.3 × 101NQ<3.3 × 100
Zenin, May 19991.1 × 1053.3 × 101NQ<3.3 × 100
Zenin, June 19991.1 × 104<3.3 × 100NQ<3.3 × 100
Zenin, July 19991.1 × 104<3.3 × 100NQ<3.3 × 100
Zenin, Oct. 19991.1 × 1053.3 × 1021.1 × 103<3.3 × 100
  • a Reported values were determined by taking into consideration that 3-liter samples were concentrated to a final volume of 1 ml (3,000× concentration factor). NQ, nonquantifiable; the sample was positive only after Southern blot hybridization of the RT-PCR product.

  • b The actual numbers of HAstV genogroup B copies in samples that also contained HAstV genogroup A were obtained by subtracting the number of genome copies estimated with primers A1/A2 (Table 1) from the numbers of genome copies estimated with primers A1, A1bis, and A2 internal.

  • c The reciprocal endpoint dilution detected by CC-RT-PCR.