TABLE 3.

MICs of control strains, fosmid clone, and subclone from the Alaskan soil metagenome for florfenicol and chloramphenicol at 30°C after 48 h of incubation

Strain (vector)Clone (mutation)MIC (μg/ml)a
ChlFfl
DH5α(pCC1FOSb)Ak20-36416
DH5α(pCC1FOS)Ak20-3 (EZ-Tn5 <KAN-2>)642
DH5α(pCC1FOS)642
DH5α(pDrive)pexA1616
DH5α42
DH5α(pDrive)42
  • a Abbreviations: Chl, chloramphenicol; Ffl, florfenicol.

  • b pCC1FOS contains a cat gene for chloramphenicol resistance.