TABLE 1.

Bacterial cloning strains, plasmids, and metagenomic clones described in this work

Strain, plasmid, gene, or cloneRelevant characteristic(s)aSource or reference
E. coli strains and plasmids for cloning
    Epi300mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1; high-transformation efficiency of large DNAEpicentre, Madison, WI
    DH5αF φ80lacZΔM15 Δ(lacZYA-argF) U169 recA endA1 hsdR17(rK mK+) phoA supE44 λ thi-1 gyrA96 relA1Invitrogen, Carlsbad, CA
    pCC1FOSChlr; fosmid cloning vectorEpicentre, Madison, WI
    pDriveAmpr Kanr; TA PCR cloning vectorQiagen, Valencia, CA
    EZ-Tn5 <KAN-2>Kanr; Tn5 for transposon mutagenesisEpicentre, Madison, WI
Metagenomic clone in pCC1FOS from Alaskan soil DNA, Ak20-3Fflr Chlr; contains a gene encoding a major facilitator superfamily drug exporterThis work
ORF of active gene from the metagenome, pexAConfers resistance to florfenicol and chloramphenicolThis work
  • a Abbreviations: Amp, ampicillin; Chl, chloramphenicol; Ffl, florfenicol; Kan, kanamycin.