TABLE 1.

Oligonucleotides used in this study

PrimerSequence (5′→3′)
DP10ghCTATTTGCAACAGTGCCGT
fabI_fwTGAAAGGTACCCATATGTCATCATGGGAATCG
fabI_revTTTTAGGTCGACGAGCCACAATTGTTAATGAG
G6PDH-NorthT7_revCTAATACGACTCACTATAGGGAGACATGTGGTTTTGCACCATATC
G6PDH-North_forGACGCGTTTATGGAACATG
gfp_fdh_fwTTATCTTGATCATAAGGGTAACTATT
gfp_fdh_revTGACACCTGATCAACTGGTAATG
Karcfw_pcx19ATTATAGATCTGAATTCAAAGATAAAAGGAGG
Komp_arc_rwATATTGAGCTCATCACCTTAAATTTTACTG
lip_fwTTAATAGGTACCTTACTATT
lip_revTCACTTAAGCGTCTGCTAAAT
Pt17_tet_fwTCAAGAGGATCCACTCCAAATATAGCT
Pt17_tet_revACTGTGAGGATCCGGATGAGTAGGTA
pxt_revGGGATCCTCTAGAGTCGACCTG
pxt_fwTCTGGTAAGCTTGAAGTTACCAC
SA0171KOP1FAAGTCGAATTCCCACAATCACAAATCATCAC
SA0171KOP2RAATATGGATCCCCCTTGAATTATTGTTAAATTC
SA0171KOP3FTATTAGTCGACGCTAGCGATTAACGCTTTC
SA0171KOP4RAATTAGATATCTGATAACGACTTGCATGCCTC
tet arc native fwATTATGCTTAGCAGACTATTTGCAACAGTGC
tet arc native revAATTTGGACCATCTGTCATGTCTATTCCTCTAGAGTCGACCTGC
tet-G6PDH_B_forTATATGTCGACCATATTGTATGACCTACTGAATGG
tet-G6PDH_B_revTATATAAGCTTTTCGTAATCAATCCTTGTCATTCG
tet-G6PDH_A_forATTATGAGCTCTGTTATTTGGCAAGCATTGTTTATTAC
tet-G6PDH_A_revTTATATCCGGACGATTCATGTATCATGTTTAATGTG
tet_pCX_fwGATCTGTACACTCGAGAGGATCCACTCCAAATATAGCT
tet_pCX_revCGAGGCATCACTTGCCGAACGGTAAGGAACCCAGACTGT