TABLE 1.

Accuracy of terminal restriction fragment length determination

Restriction enzymeT-RF length (nt)a
Calculated from sequence dataWith ROX-labeled markerbWith 6-FAM-labeled markerb
AluI7266.6 ± 0.272.0 ± 0.1
HhaI207205.0 ± 0.2207.3 ± 0.1
MspI490489.4 ± 0.1490.9 ± 0.2
RsaI878880.7 ± 0.8883.3 ± 0.8
  • a The fragment used was a P. stutzeri strain KC 6-FAM-labeled 16S rRNA gene fragment. The data are the averages of eight independent replicates.

  • b The marker was the MapMarker 1000 internal size standard (50, 75, 100, 125, 150, 200, 250, 300, 350, 400, 450, 475, 500, 550, 600, 650, 700, 750, 800, 850, 900, 950, and 1,000 nt).