TABLE 2.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant characteristic(s)Reference
B. subtilis
    WH557trpC2 lacA::Pt17-tetR lox66-aphAIII-lox719
    WH557-gfptrpC2 lacA::Pt17-tetR lox66-aphAIII-lox71 amyE::Pxyl/tet-gfpmut29
    WH558trpC2 lacA::Pt17-tetR lox66-aphAIII-lox71 amyE::Pxyl/tet-lacZ9
E. coli
    DH5αλ φ80dlacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rK mK) supE44 thi-1 gyrA relA126
    XL1-BluehsdR17(rK mK+) recA1 endA1 gyrA96 thi-1 supE44 relA1 lac [F′ proAB lacIq ZΔM15 Tn10 (Tetr)]Stratagene
S. aureus
    SA113 (ATCC 35556)NCTC8325 derivative; agr negative; 11-bp deletion in rbsU29
    RN4220NCTC8325-4 derivative; acceptor of foreign DNA29
    RAB133arcABDCR::lox7240
    RAB171SA113 fdh::Pxyl/tet-gfpmut2This work
    RAB180RN4220 lip::Pt17-tetR lox66-aphAIII-lox71This work
    RAB190RN4220 lip::Pt17-tetR lox72This work
    RAB200SA113 lip::Pt17-tetR lox66-aphAIII-lox71This work
    RAB210SA113 lip::Pt17-tetR lox72This work
    RAB211SA113 lip::Pt17-tetR lox72 fdh::Pxyl/tet-gfpmut2This work
    RAB215SA113 lip::Pt17-tetR lox66-aphAIII-lox71 Pxyl/tet-zwfThis work
    RAB216SA113 lip::Pt17-tetR lox72 Pxyl/tet-zwfThis work
    RAB220SA113 lip::Pt17-revtetR lox66-aphAIII-lox71This work
    RAB230SA113 lip::Pt17-revtetR lox72This work
    RAB231SA113 lip::Pt17-revtetR lox72 fdh::Pxyl/tet-gfpmut2This work
    RAB235SA113 lip::Pt17-revtetR lox66-aphAIII-lox71 Pxyl/tet-zwfThis work
    RAB236SA113 lip::Pt17-revtetR-r2 lox72 Pxyl/tet-zwfThis work
S. carnosus
    TM300Wild type50
Plasmids
    pBT2cat bla; E. coli/Staphylococcus shuttle vector; thermosensitive ori for staphylococci10
    pCX19cat xylR lip; Staphylococcus vector28
    pIC156Source of specR52
    pRAB1cat bla PpagA-cre; pBT2 derivative40
    pRAB2(-r2)cat blalip′-Pt17-(rev-)tetR-lox66-aphAIII-lox72-lip′; pBT2 derivativeThis work
    pRAB4cat blaSAOUHSC_00139-141-Pxyl/tet-gfpmut2-specR-SAOUHSC_00143′; pBT2 derivativeThis work
    pRAB6cat bla ble Pxyl/tet-AS ′fabI′; pRB473 derivativeThis work
    pRAB7(-r2)cat Pt17-(rev-)tetR; pCX19 derivativeThis work
    pRAB8cat PR*-tetR Pxyl/tet-arcABDCR; pCX19 derivativeThis work
    pRAB9cat bla SAOUHSC_01599-lox66-aphAIII-lox72-Pxyl/tet-zwf; pBT2 derivativeThis work
    pRB473cat bla ble; E. coli/Staphylococcus shuttle vector11
    pWH105-gfpmut2blaamyE′-cat-Pxyl/tet-gfpmut2-′amyE′; pWH105 derivativeThis work
    pWH1925-r2Source of revtetR51
    pWH1935-1aSource of tetR9
    pWH1935-2Source of Pxyl/tet9