TABLE 2.

Plasmids used and constructed in this study

PlasmidDescriptionSource or reference
pUC18ori ColE1 (Apr)Pharmacia
pKOBEGApSC101ts::PBADredγδβα (Apr)10
pSU18ori p15A (Cmr)1
PBADgfpgfp cassette (S65T, F64L) (Apr)J. M. Ghigo
PSU18T-PBADgfppSU18::oriTRP4-araC-PBADgfp (Cmr)This study
PSU18T-PBADgfp2pSU18::oriTRP4-araC-PBADgfp (Cmr)This study
PSU18T-PBADvsmpSU18::oriTRP4-araC-PBADvsm (Cmr)This study
pSW23ToriVR6Kγ oriTRP4 (Cmr)17
pSW29ToriVR6Kγ oriTRP4 (Kmr)17
pSW4426TpSW23T::aadA7-araC-PBADccdB (Spr Cmr)This study
pSW4427TpSW29T::aadA7-araC-PBADccdB (Spr Kmr)This study
pSWδ2989TpSW4426T, Δvsm (Spr Cmr)This study
pSWδ4175TpSW4426T, ΔluxU (Spr Cmr)This study
pSWδ3453TpSW4426T, ΔluxM (Spr Cmr)This study
pSWδ5679TpSW4426T, ΔgyrA (Spr Cmr)This study
pSWδintIATpSW4427T, ΔintIA (Spr Kmr)This study
pSWδ6720T-vsmpSW4426T, ΔISVs1 orfB::araC-PBADvsm (Spr Cmr)This study