TABLE 1.

FISH probes used in this study

ProbeSequence (5′-3′)% FormamideLabelTarget specificityReference
EUB338aGCT GCC TCC CGT AGG AGT0-50FITCMost Bacteria2
EUB338-IIaGCA GCC ACC CGT AGG TGT0-50FITCPlanctomycetales10
EUB338-IIIaGCT GCC ACC CGT AGG TGT0-50FITCVerrucomicrobiales10
ARCH915GTG CTC CCC CGC CAA TTC CT20Cy3Most Archaea58
EP404AAA KGY GTC ATC CTC CA30Cy3Most Epsilonproteobacteria39
GAM42abGCC TTC CCA CAT CGT TT35Cy3Most Gammaproteobacteria40
DELTA495acAGT TAG CCG GTG CTT CCT45Cy3Most Deltaproteobacteria, some Gemmatimonas group35
SRB385CGG CGT CGC TGC GTC AGG35Cy3Some Deltaproteobacteria, some Actinobacteria, and Gemmatimonas group3
  • a EUBMIX is a mixture of EUB338, EUB338-II, and EUB338-III.

  • b Requires competitor oligonucleotide cGam42a: GCCTTCCCACTTCGTTT.

  • c Requires competitor oligonucleotide cDELTA495a: AGTTAGCCGGTGCTTCTT.