TABLE 1.

Primers used to amplify genetic elements

FragmentPrimer namePrimer sequencea
flgB promoterpflgB1-forward5′-CTGCAG TAATACCCGAGCTTCAAG-3′
pflgB2-reverse5′-CATATGACCTCCCTCATTTAAAATTGC-3′
cheA2FPA2-forward5′-CATATGATTAAAGAGGAGAAATTAACTATG-3′
RPA2-reverse5′-GCATGCTCAGCTAATTAAGCTTGGCTG-3′
T7 terminatorpFT7-forward5′-GCATGCGCTAACAAAGCCCGAAAGGAAGCT-3′
pRT7-reverse5′-AAGCTTTGCAGATCCGGATATAGTTCCTCC-3′
cheA2 probeDFA2-forward5′-AAAAGACAATCCTATGGCTAC-3′
DFA2-reverse5′-TAACTCTCTCTTCAACTGTTTC-3′
  • a Engineered restriction sites are underlined.