TABLE 2.

Divergence in 16S rRNA sequence among putative ecotypes and recognized taxa

Putative ecotypeDivergencea
B. subtilis subcladebB. licheniformis subcladec
PE 12PE 18PE 19PE 14PE 10PE 21PE 23PE 15PE 11PE 9BPE 9APE 5PE 2PE 31PE 29PE 4
PE 12 0.18 0.08 0.28 0.08 0.08 0.15 0.15 0.08 0.11 0.17 1.561.231.581.441.51
PE 18 0.18 0.67 0.18 0.18 0.18 0.36 0.18 0.36 0.36 1.461.461.461.461.46
PE 19 0.19 0.14 0.14 0.21 0.07 0.00 0.21 0.25 1.711.301.641.511.58
PE 14 0.09 0.34 0.32 0.39 0.25 0.19 0.39 0.43 1.901.491.831.691.76
PE 10 0.00 0.07 0.21 0.14 0.21 0.25 1.711.301.641.511.58
PE 21 0.07 0.21 0.14 0.21 0.25 1.711.301.641.511.58
PE 23 0.27 0.21 0.27 0.32 1.651.371.711.581.64
PE 15 0.07 0.14 0.19 1.781.371.711.581.64
PE 11 0.14 0.19 1.631.361.711.571.64
PE 9b 0.05 1.781.371.711.581.64
PE 9a 0.04 1.831.421.761.621.69
PE 5 0.04 0.55 0.20 0.34 0.28
PE 2 0.34 0.48 0.27
PE 28 0.14 0.07
PE 27 0.21
PE 4
  • a Comparisons within the B. subtilis subclade and within the B. licheniformis subclade are given in boldface. —, only one strain was sequenced from the ecotype, and therefore, the within-ecotype divergence is not indicated. From RFW isolates, 1,460 bp was sequenced; from other strains, 1,466 bp was available from the All Species Living Tree Project, with the exceptions of 1,169 bp for B. subtilis subsp. inaquosorum and 548 bp for B. tequilensis (63).

  • b PE 12 is B. subtilis subsp. inaquosorum; PE 18 is B. tequilensis; PE 14 is B. vallismortis; PE 10 is B. subtilis subsp. subtilis; PE 15 and PE 11 are B. subtilis subsp. spizizenii; and PE 9B and PE 9A are B. mojavensis.

  • c PE 5 is B. licheniformis.