TABLE 2.

Mutations in the reporter strains

StrainSequenceaMutationPrimers
NZ7100ATTGATTATATC AAG TGG GAT ATGAACWild typeb
NZ1801ATTGATTATATC TAG TGG GAT ATGAACSubstitution stop codon1L, 1R
NZ1802ATTGATTATATC AAG TGG TAT ATGAACSubstitution Asp → Tyr2L, 2R
NZ1803ATTGATTATATC AAG GG GAT ATGAAC1-bp deletion frameshift3L, 3R
NZ1804ATTGATTATATC AAG G GAT ATGAAC2-bp deletion frameshift4L, 4R
NZ1805ATTGATTATATC GAAG TGG GAT ATGAAC1-bp insertion frameshift5L, 5R
NZ1806ATTGATTATATC AAG TGG CTG ATATGAAC2-bp insertion frameshift6L, 6R
NZ7100AAGTTACCA GGA GGC TTAGCGGATATTAGWild typec
NZ1807AAGTTACCA GGG GGGG TTAGCGGATATTAG1-bp insertion frameshift7L, 7R
  • a Underlining indicates the catalytic site of the melA gene; bold type indicates the mutation site.

  • b Positions 1438 to 1464 of the melA gene.

  • c Positions 1189 to 1216 of the melA gene.