TABLE 6.

Genes outside the nitrogenase gene cluster expressed at higher levels in the hydrogen-producing mutants

Locus tag(s)aGeneAvg fold difference in expressionbAnnotation
CGA570CGA571CGA572CGA574CGA584Wild type (NF)c
RPA1848-RPA18491.13.02.42.12.52.5Uncharacterized iron-regulated membrane protein/conserved hypothetical protein
RPA1927-RPA192820667.0363834Uncharacterized conserved protein/ferredoxin-like protein [2Fe-2S]
RPA215614534.9193332Conserved hypothetical protein
RPA2380-RPA23882.68.51.03.04.810Iron(III) transport
RPA3012-RPA3013pucABd3.33.62.23.85.16.1Light-harvesting proteins B-800-850 (LHII)
RPA47149.240.32.7221719Conserved hypothetical protein
RPA48273.8151.77.08.67.3Conserved hypothetical protein
  • a Genes forming putative operons are grouped, and the values shown represent the gene with the highest average expression ratio.

  • b Cells were grown anaerobically in light with acetate and ammonium sulfate. The differences are relative to the values for the wild type grown anaerobically in light with acetate and ammonium sulfate.

  • c Gene expression differences of the wild type (CGA009) grown with acetate under nitrogen-fixing conditions (NF) are shown for comparison relative to cells grown with acetate and ammonium sulfate.