TABLE 3.

Physical properties for PHB extracted from bioreactor cultures of recombinant E. coli strains grown on glycerol or glucose at high or low aeration

StrainaCulture conditionMrb (MDa)Tgc (°C)Tmd (°C)% Crystallinity
K24KPGlycerol, 125 rpm1.90 ± 0.057.0 ± 0.1175.1 ± 0.157.3 ± 1.0
Glycerol, 500 rpm1.71 ± 0.054.9 ± 0.1173.1 ± 0.152.6 ± 1.0
Glucose, 125 rpm2.21 ± 0.053.1 ± 0.1177.0 ± 0.160.8 ± 1.0
Glucose, 500 rpm2.28 ± 0.055.1 ± 0.1177.3 ± 0.161.7 ± 1.0
Glycerol, 1,000 rpm2.04 ± 0.05NDe177.3 ± 0.566.4 ± 1.5
K24KvcGlycerol, 1,000 rpm1.80 ± 0.05ND169.8 ± 0.452.6 ± 0.4
  • a K24KP carries phaBAC and phaP of Azotobacter sp. FA8. K24Kvc carries phaBAC of Azotobacter sp. FA8.

  • b M r, relative molecular mass.

  • c T g, glass transition temperature.

  • d T m, melting temperature.

  • e ND, not determined.