TABLE 4.

Growth response of select gene deletion strains in E. coli DH1 (0.8% n-butanol exposure)

StrainaMean difference (SE) in OD600/ml atb:
4 h10 h
DH10.13 (0.01)0.47 (0.04)
BW251130.26 (0.04)0.48 (0.05)
    Δdcp0.19 (0.01)0.59 (0.04)
    ΔmobB0.24 (0.03)0.29 (0.06)
    ΔevgS0.13 (0.00)0.75 (0.05)
    ΔcpxP0.060 (0.02)0.38 (0.01)
    ΔmalE0.34 (0.01)0.62 (0.03)
    ΔoppA0.23 (0.02)0.33 (0.06)
    ΔsodC0.13 (0.02)0.34 (0.07)
    ΔyqhD0.34 (0.02)0.44 (0.05)
    ΔenvY0.022 (0.01)0.36 (0.03)
    ΔmetE0.22 (0.03)0.27 (0.08)
    ΔompF0.33 (0.01)0.43 (0.03)
  • a Strains were obtained from the Keio Collection (Japan). The background strain for the Keio knockouts (BW25113) was obtained from the Yale culture collection.

  • b Difference [control (0% n-butanol) − stress (0.8% n-butanol)] in optical density (OD600) for a given strain. The time points are post n-butanol addition. Assays were conducted in triplicate in 24-well plates using the Tecan Safire plate reader in M9 medium.