TABLE 1.

Fermentation parameters for 24-h bioreactor cultures of strain K24KP grown in glucose or glycerol under different aeration conditions

Culture conditionCDWa (g·liter−1)% PHBb (wt/wt)μmaxc (h−1)O2 consumptiond (μmol O2·g CDW−1·h−1)qsubstratee (mmol·g CDW−1·h−1)
Glycerol
    125 rpm4.75 ± 0.0530.1 ± 0.40.69 ± 0.029.6 ± 0.51.86 ± 0.12
    500 rpm9.62 ± 0.0216.9 ± 0.30.79 ± 0.028.9 ± 1.31.05 ± 0.04
Glucose
    125 rpm4.45 ± 0.0220.4 ± 0.10.75 ± 0.034.9 ± 0.51.41 ± 0.02
    500 rpm9.43 ± 0.0137.2 ± 0.50.89 ± 0.045.1 ± 0.40.75 ± 0.18
  • a CDW, cell dry weight.

  • b The amount of PHB is given as a percentage of the weight (average ± standard deviation) of the CDW.

  • c Maximum specific growth rate.

  • d Oxygen consumption was determined as indicated in reference 19.

  • e The specific consumption rate was calculated on the basis of glucose or glycerol consumption.