TABLE 4.

Mass spectrometry identification of H. volcanii proteins obtained by immobilized metal affinity chromatography

Protein nameNCBI or Swiss-Prot entryMASCOT scoreCoverage (%)No. of unique peptidesPeptide sequencesPredicted mass (kDa)Observed mass (kDa)HaloLex entry
PitAgi‖224820302 (Natrialba magadii chlorite dismutase)6332SIDWDAWR56.160.9HVO_1871
DVLADRPR
Cdc48dgi‖110668990 (Haloquadratum walsbyi AAA-type ATPase)369125ILFVGPPGTGK53.455.7HVO_1907
EAVLEALTEER
ILFIDEFDSVAKa
LSMITSQYLGETAK
RFDEIVNFPKPDR
RadAQ48328 (Haloferax volcanii DNA repair and recombination protein RadA)546329ADIGSSTASDIINAA38.3b41.9HVO_0104
DAADVGGFETGSMVLER
GLEDEALEATLDDR
EMEGSIDDEETIK
ALVDDFLDK
VDDFLDKIHVAKa
AGEHEDTEWPVRa
LVDAPNLADGEAIMR
VQDAGLKPE
  • a Semitryptic peptide.

  • b Including the hexahistidine tag.