TABLE 2.

Replication functions of pRSB105 Rep1 and Rep2 in different proteobacteria

ConstructSubcloned pRSB105 replication moduleCharacteristicsPresence (+) or absence (−) of replication ability in:
S. meliloti, A. tumefaciens (α-proteobacteria)R. eutropha (β-proteobacterium)X. campestris pv. campestris (γ-proteobacterium)
pNK100-Rep1aRep1Unknown Inc group, pRSB101-likec+
pIK100-Rep2bRep2IncP-6, Rms149-liked++
  • a The pRSB105 Rep1 module was cloned on a 4,673-bp SacII fragment into the mobilizable suicide plasmid pK18-mob.

  • b The pRSB105 Rep2 module was cloned on a 8,782-bp PstI fragment into the mobilizable suicide plasmid pSUP202.

  • c The erythromycin resistance plasmid pRSB101 was isolated from an unknown activated sludge bacterium (47).

  • d The multiresistance plasmid Rms149 was isolated from a clinical P. aeruginosa strain (20).