TABLE 3.

Experimental conditions for studies assaying survival of enteric viruses on fomites

Virus (reference[s])Suspension mediumFomiteTemp (°C)% HumidityPBS or BEMaFSb
Survival (days)T90 (days)T99 (days)KicT90 (days)T99 (days)Kic
Hepatitis A virus (1, 48)PBS or FSAlum.d485-90>6035>600.0027845>600.00278
Alum.2085-90>6035>600.0027835>600.00278
Alum.2045-55>6011500.0027815600.00278
Paper485-90>60<150.00278<170.00278
Paper2085-90>60<120.00278<160.00278
Paper2045-55>60<110.00278<140.00278
Adenovirus 40 (1)PBS or FSAlum.485-9015<1<10.011<1<10.00278
Alum.2085-9015<1<10.011<1<10.00278
Alum.2045-5515<1<10.011<1<10.083
Paper485-90>30<110.00278<110.011
Paper2085-90>30<110.00278<110.066
Paper2045-55>30<110.00278<110.066
Rotavirus p13 (1)PBS or FSAlum.485-90>60<1>600.00278<1110.00278
Alum.2085-90>60<1500.0027814>600.00278
Alum.2045-55>60<1110.0027815>600.00278
Paper485-90>60<1>500.00278<1150.00278
Paper2085-90>60<1>600.00278<1>600.00278
Paper2045-55>60<12.50.00278<1180.00278
Astrovirus (type 4) (2)PBS or FSChina485-9560<110.00218150.0021
China207<1<10.025<150.025
Paper4905150.0014190.0014
Paper2060<1<10.0021<1<10.0138
Feline calicivirus F9 (22)BEMGlass coverslip4NDe5710100.0059
Glass20ND35<1100.0119
Glass37ND7<110.33
  • a PBS, phosphate buffer solution (for all viruses except feline calicivirus F9); BEM, basal Eagle's medium (used only for feline calicivirus F9).

  • b FS, 20% fecal suspension.

  • c Inactivation coefficient (Ki) = log10 reduction in virus titer per ml = (initial viral titer − final viral titer)/survival (in hours) (64).

  • d Alum., aluminum surface.

  • e ND, not determined.