TABLE 2.

Comparison of standard plating and magnetic separation with CBD118-coated beads for detection of L. monocytogenes EGDe in artificially contaminated food samples

Food sample and initial contamination (CFU/g)Result obtained with following conditions for selective enrichment culturea
6 h24 h48 h
IDF/ISObCBD-MSIDF/ISOCBD-MSIDF/ISOCBD-MS
Sliced iceberg lettuce
    0
    0.1++++++++++++
    1(+)++++++++++++
    10+++++++++++++
    100++++++++++++++
Camembert soft cheese
    0
    0.1+
    1+++++++++
    10++++++++++++
    100+++++++++++++++
Red smear cheese
    0
    0.1++
    1(+)+++
    10++++++++++++
    100+++++++++++++
Smoked salmon
    0
    0.1+++++++
    1+++++++++
    10+++++++++++++
    100+++++++++++++++
Minced meat
    0
    0.1+++++++++
    1+++++++++++
    10+++++++++++++
    100++++++++++++++
Turkey breast
    0
    0.1+++++
    1+++++++++++
    10+++++++++++++
    100++++++++++++++
Milk (pasteurized)
    0
    0.1++++++++++++
    1++++++++++++
    10+++++++++++++
    100+++++++++++++++
  • a The number of plus signs indicates the number of typical Listeria colonies on Oxford agar as follows: (+), presence of a single colony on one plate; +, 1 to 10 colonies; ++, 10 to 50 colonies; +++, more than 50 colonies.

  • b See Materials and Methods for details.