TABLE 1.

Comparative summary of DNA extraction methods and spiked experimentsa

DNA extraction methodDNA yield (ng ml−1)Total copy no. (103 ml−1) in:% Recovery in seeded samples
Seeded samplesNonseeded samples
FastDNA1.40 (0.15)88.8 (28.6)2.3 (0.65)10.7 (3.5)
UltraClean0.52 (0.04)60.5 (2.91)9.6 (2.68)6.3 (0.1)
Phenol-chloroform1.56 (0.22)180.4 (9.61)32.5 (8.43)18.7 (0.5)
  • a Data are means (SEs) for four replicates. Copy numbers were calculated by the volume-based method (equation 1).