TABLE 3.

Evaluation of downstream purification steps

TreatmentAmt (%) of DNA recovereda with the following amt of DNA added per sample:
20 μg50 μg100 μg300 μg
Ethanol7.5 ± 6.7 (37.4)32.6 ± 21.2 (65.1)67.1 ± 37.5 (67.1)197.5 ± 75.5 (65.8)
Ethanol + GlycoBlue17.5 ± 7.6 (87.4)38.8 ± 17.4 (77.5)72.0 ± 32.2 (72.0)198.3 ± 79.0 (66.1)
Spin column13.6 ± 0.5 (68.1)39.3 ± 1.1 (78.7)71.8 ± 2.3 (71.8)237.0 ± 5.4 (79.0)
  • a Amounts of DNA recovered are expressed as micrograms per sample and are means ± SEs for three replicates.