Amplification of 16S rRNA genes using real-time PCRa

DNA extraction methodBacterial 16S rRNA gene copy no. (106):
Per ml of seawaterPer ng of DNA
FastDNA2.7 (0.099)0.79 (0.086)
UltraClean0.91 (0.025)1.4 (0.063)
Phenol-chloroform3.0 (0.053)1.5 (0.065)
  • a Data are calculated and presented as the numbers of copies per milliliter of seawater (Nvol method; equation 1) and per nanogram of DNA (Nwt method; equation 2). Data are means (SEs) for four replicates.