TABLE 2.

Cell and 16S rRNA gene copy concentrations in the six biogas reactors determined by FISH and Q-PCR

Biogas plantTotal cell count (ml−1)aBacterial abundance determined by:Archaeal abundance determined by:
FISH (cells ml−1)Q-PCR (16S rRNA gene copies ml−1)FISH (cells ml−1)Q-PCR (16S rRNA gene copies ml−1)
R1(8.5 ± 5.6) × 108(6.2 ± 2.7) × 108(2.5 ± 0.4) × 1011(6.5 ± 5.8) × 107(2.0 ± 0.3) × 1010
R2(3.1 ± 2.5) × 108(9.6 ± 7.0) × 107(9.3 ± 2.8) × 1011(1.7 ± 1.4) × 107(3.1 ± 0.2) × 1010
R3(7.6 ± 0.0) × 108(4.6 ± 2.3) × 108(9.6 ± 3.7) × 1010(3.0 ± 1.2) × 107(5.0 ± 0.8) × 109
R4(1.9 ± 5.4) × 108(1.0 × 0.2) × 109(1.2 ± 0.6) × 1011(7.9 ± 2.9) × 107(3.0 ± 0.9) × 109
R5(1.1 ± 1.0) × 108(5.6 ± 4.1) × 108(4.1 ± 0.9) × 1011(7.8 ± 6.0) × 107(2.9 ± 0.9) × 1010
R6(1.9 ± 8.7) × 108(9.8 ± 5.3) × 108(2.5 ± 0.2) × 1011(1.3 ± 0.9) × 108(2.1 ± 0.7) × 1010
  • a Detected by DAPI staining.