TABLE 1.

Solvent stabilities of spore-displayed β-Gal on different B. subtilis spores and free-form enzymes in various organic solvents after 1 h of incubation at 37°C

SolventSolvent hydrophobicity (log P value)Relative activities of β-Gal
Displayed on DB104 sporesDisplayed on WB700 sporesFree form
Control100100100
n-Hexane3.510010084.3
Ethyl ether0.877.297.048.2
Toluene2.551.678.84.2
Ethyl acetate0.689.610.60.1
Acetonitrile−0.330.88.30.0
Ethanol−0.240.00.00.0