TABLE 1.

Primers used for HXT3 cloning and integration in V5 hxt1-7Δ

PrimerSequence (5′ to 3′)aLocalization
HXT3P1GTGCGGGATccGAAGGCAATATC−1128
HXT3P2gatcggATCCATCATCACGTTCCTAGC2096
I2HXT3 aagtgacgggcgatgagtaagaaagaaataactgactcattagaCCATCATCACGTTCCTAGC2095
C1HXT3ORFGACACAGTGACATATGCACC168
C2HXT3pTTAAGCATGATCGTCTAGGC−1689
HXT3p426 Aacacaaaaacaaaaagtttttttaattttaatcaaaaa CTGAGTTAAACAATCATGAATTCAACTCC−15
HXT3t426 GaatgtaagcgtgacataactaattacatgactcgagACGGTTTAGCGTGAAATTATTTCTTGCC1694
C1HXT3ORFGACACAGTGACATATGCACC168
C′2HXT7p426 gccaatacttcacaatgttcg −125
  • a Underlining indicates homology with the HXT7 terminator, uppercase letters indicate homology with HXT3, double underlining indicates homology with the p4H7 promoter, italics indicate homology with the p4H7 terminator, and boldface type indicates homology with the HXT7 promoter.