TABLE 1.

Primers used in this study

NameSequence (5′-3′)Taa (°C)Product sizeApplicationcReference
Primers for PR genes
    PR1MGN TAY ATH GAY TGG YT47312 bpScr32
    PR2WWN MGN TAY GTN GAY TGG
    PR3GGR TAD ATN GCC CAN CC
    PR4GTC AGG CTG GTC TTA CGT AGA CTG66NAbChrwThis study
    PR5TTA CTC GCC GAC CCT ACA AAG CTA65NAChrwThis study
    PRO95_REV_68GCA GAA GCT GCC ATC ATA GC62NAChrwThis study
    PRO95_REV_395ATA GCT CCC CAT AAT GCT GC60NAChrwThis study
    PRO95_PR_FCCC CAT ATG AAA TTT TTA TTA C53756 bpConThis study
    PRO95_PR_RCCC CAT ATG TTA AAC AAC C56
    PRO95_FORCGT TCC ATT AAT GTG CGT TG59158 bpqRT-PCRThis study
    PRO95_REVTAG CTC CCC ATA ATG CTG CT
    SARPR_125FTHG GWG GAT AYT TAG GWG AAG C54163 bpqPCR6
    SARPR_288RCCC AAC CWA YWG TWA CRA TCA TTC T
    Flavo_F2GGA TAC TAA CAG TTC CWT TRA TGT GTG56qPCR6
    Flavo_R2CCR AAG TAA CCW GTR ACA AGC AT
    HTCC2255_FATG CTC GTA GGT GGG TAT GC55qPCRThis study
    HTCC2255_RGCC TCA TCT GCA GCA ACT TT
    MEDHO_fTGC CGC AGT CTC YTC WAG TTC55qPCRThis study
    MEDHO_rGAA TCC TAG GCT WGC YGA CAT
    MS0242_FTTA CTG TGC CAT TAA TGT GTG TTG55qPCRThis study
    MS0242_RAAT CCA GAA GTA TGC AAG ACC TG
    Flavo-H_fCTA ACW GTD CCW TTR ATG TGT GTT G55qPCRThis study
    Flavo-H_rCCT GTW GTG TAW ACW GCT TCT CC
Primers for 16S rRNA genes
    16F27AGA GTT TGA TCC TGG CTC AG541.5 kbPhy22
    16R1492TAC GGY TAC CTT GTT ACG ACT T
    BACT1369FCGG TGA ATA CGT TCY CGG58174 bpqPCR38
    PROK1543RAAG GAG GTG ATC CR GCC GCA
    PRO95_16SFTGC GAT AGA TAG GGG TCC TG59150 bpqRT-PCRThis study
    PRO95_16SRACC CAT AGG GCA GTC TTC CT
  • a Ta, annealing temperature.

  • b NA, not applicable.

  • c Application of the primer: scr, screening; chrw, chromosomal walking; con, confirmation of PR gene sequence (amplification of the complete PR gene from D. donghaensis PRO95); qPCR, quantitative PCR; and phy, phylogenetic analysis.