TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidDescriptionaReference or source
Escherichia coli strains
    HB101supE44 Δ(mcrC-mrr) recA13 ara-14 proA2 lacY1 galK2 rpsL20 xyl-5 mtl-1 leuB6 thi-114
    DH5αF φ80dlacZΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 hsdR17(rK mK+) phoA supE44 λ thi-1 gyrA96 relA1Invitrogen
Bacteroides strains
    B. fragilis NCTC9343Type strain, appendix abscessNCTC
    B. fragilis YCH46Clinical isolate, blood12
    B. thetaiotaomicron VPI-5482Human fecesVPI Anaerobe Laboratory
    B. vulgatus ATCC8482Human fecesATCC
Plasmids
    pBlueScript KS II(+)Cloning vector, AprStratagene
    pVAL-1Apr Tcr in E. coli, Emr in Bacteroides; Mob+ Rep+24
    pVAL-2Apr in E. coli, Cfxr in Bacteroides; Mob+ Rep+This study
    pLYL05Apr in E. coli, Cfxr in Bacteroides; Mob+ Rep+18
    pNLY1Apr in E. coli, Cmr in Bacteroides; Mob+ Rep+18
    pLYL0519Flanking region (2 kb each) of Tsr19 gene deletion site was cloned into pLYL05; Cfxr Mob+ Rep+This study
    pLYL0520Targeting vector for deletion of Tsr19 gene in strain NCTC9343This study
  • a Abbreviations: Ap, ampicillin; Tc, tetracycline; Em, erythromycin; Cfx, cefoxitin; Mob, ability to be mobilized by a conjugative element; Rep, ability to replicate in Bacteroides.