TABLE 2.

Genes constitutively upregulated in the Tcsr mutants compared to the level for the parent strain R. rubrum S1Ha

StrainTCS MIC (mg/liter)Fold induction ratio for:
Micropollutant-upregulated factorsemrAemrBMultidrug efflux pump genesRru_A1250EPS synthesis genesgpoPhage shock protein genesatoE
mufA1mufMmexAmexBmexDmexFcapDexoDpspApspC
M260.075351.9 ± 154.925.9 ± 5.79.1 ± 1.52.6 ± 0.211.7 ± 2.05.2 ± 1.6
M170.1002.3 ± 1.03.5 ± 0.32.5 ± 0.814.5 ± 2.34.3 ± 1.53.2 ± 0.2
M30.1003.0 ± 1.92.3 ± 0.53.8 ± 0.92.6 ± 1.52.5 ± 0.4
M130.100305.8 ± 56.623.4 ± 3.313.5 ± 5.62.6 ± 1.83.4 ± 0.44.1 ± 0.92.7 ± 1.53.5 ± 2.82.0 ± 1.12.6 ± 0.5
M270.100141.6 ± 45.015.7 ± 4.311.7 ± 2.42.9 ± 0.82.4 ± 0.5
M210.2502.8 ± 0.311.7 ± 7.42.2 ± 0.22.3 ± 2.219.4 ± 2.54.0 ± 0.22.5 ± 0.32.4 ± 0.73.4 ± 1.3
M280.250143.4 ± 20.38.1 ± 1.88.3 ± 2.93.0 ± 0.82.0 ± 0.62.6 ± 1.0
M340.500188.6 ± 51.717.8 ± 3.013.4 ± 2.52.9 ± 0.82.9 ± 0.3
M331.0003.4 ± 0.72.4 ± 0.12.5 ± 0.3
M368.000
M36+>16.0002.6 ± 0.51.9 ± 0.4
  • a Values are shown for genes with relative fold induction ratios of ≥2.0. The standard deviations for the biological triplicates are given. The genes fabI1, fabI2, ompC, ompF, acrC, phaS, and Rru_A1640 are not included, since they were not found significantly upregulated in any mutant. A minus sign indicates that the gene was not significantly (≥2.0-fold) upregulated. Bold indicates a P value of ≤0.05.