TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmid and functionRelevant genotype or descriptionaReference or source
Bacterial strains
    Escherichia coli
        DH5α-λpirφ80dlacZΔM15 Δ(lacZYA-argF)U196 recA1 hsdR17 deoR thi-1 supE44 gyrA96 relA1pir48
        WM3064thrB1004 pro thi rpsL hsdS lacZΔM15 RP4-1360 Δ(araBAD)567 ΔdapA1341::[erm pir(wt)]W. Metcalf, University of Illinois, Urbana-Champaign
        BTH101aF cya-99 araD139 galE15 galK16 rpsL1 (Strr) hsdR2 mcrA mcrB1Euromedex, France
    Shewanella oneidensis MR-1
        S79Wild type72
        S143ΔSO_0577 (ΔarcS)This study
        S838Wild type (“knock-in” complementation of ΔarcS)This study
        S313ΔSO_1327 (ΔhptA)This study
        S802Wild type (knock-in complementation of ΔhptA)This study
        S318ΔSO_3988 (ΔarcA)This study
        S805Wild type (knock-in complementation of ΔarcA)This study
        S315Δarc ΔhptAThis study
        S320ΔarcS ΔarcAThis study
        S836ΔhptA ΔarcAThis study
        S834ΔarcS ΔhptA ΔarcAThis study
Plasmids
    pNPTS138mobRP4+ sacB ColE1 ori KmrM. R. Alley, unpublished data
    pUC18R6KT-mini-Tn7TmobRP4+ ori-R6K TnR/TnL Apr13
    pNPTS138-R6KTmobRP4+ ori-R6K sacB; suicide plasmid for in-frame deletions; KmrThis study
    Construction of in-frame deletions and complementation in Shewanella sp.
        pGP704Sac28KmmobRP4+ ori-R6K sacB; suicide plasmid for in-frame deletions; KmrChengyen Wu, unpublished data
        pGP704Sac28Km-ΔSO_0577SO_0577 (arcS) deletion fragment in pGP704Sac28KmThis study
        pGP704Sac28Km-KI-SO_0577SO_0577 (arcS) complementation fragment in pGP704Sac28KmThis study
        pNPTS138-R6KT-ΔhptAhptA deletion fragment in pNPTS138R6KTThis study
        pNPTS138-R6KT-KI-hptAhptA complementation fragment in pNPTS138R6KTThis study
        pNPTS138-R6KT-ΔarcAarcA deletion fragment in pNPTS138R6KTThis study
        pNPTS138-R6KT-KI-arcAarcA complementation fragment in pNPTS138R6KTThis study
        pNPTS138-R6KT-ΔSputCN32_3300SputCN32_3300 (arcS) deletion fragment in pNPTS138R6KTThis study
        pNPTS138-R6KT-KI-SputCN32_3300SputCN32_3300 complementation fragment in pNPTS138R6KTThis study
        pBAD33ori-p15a araC PBAD Cmr23
        pBAD33-RBS-SO0577SO_0577 (arcS) in pBAD33This study
        pBAD33-RBS-hptAhptA in pBAD33This study
        pBAD33-RBS-arcAarcA in pBAD33This study
    Overproduction of S. oneidensis MR-1 Arc components
        pBAD-HisAInvitrogen
        pBAD-HisA-SO_0577-646SO_0577 C-terminal coding region (aa 646-1188) in pBADHisAThis study
        pBAD-HisA-arcAarcA in pBADHisAThis study
        pBAD-HisA-arcA-D54NarcA(D54N) in pBADHisAThis study
        pBAD-HisA-SO_3457-181SO_3457 C-terminal coding region (aa 181-929) in pBADHisAThis study
        pGEX4T-1ori-pBR322 Plac Apr
        pGEX4T-1-hptAhptA in pGEX4T-1This study
        pGEX4T-1-hptA-H62AhptA(H62A) in pGEX4T-1This study
    Bacterial two-hybrid constructs
        pUT18ori-ColE1 Plac T18 for N-terminal fusion; AprEuromedex, France
        pUT18Cori-ColE1 PlacT18 for C-terminal fusion; AprEuromedex, France
        pKT25ori-p15a Plac T25 for C-terminal fusion; KmrEuromedex, France
        pKNT25ori-p15a Plac T25 for N-terminal fusion; KmrEuromedex, France
        pUT18-SO_0577-364SO_0577 (arcS) C-terminal region (aa 364-1188) in pUT18This study
        pUT18C-SO_0577-364SO_0577 (arcS) C-terminal region (aa 364-1188) in pUT18CThis study
        pKT25-SO_0577-364SO_0577 (arcS) C-terminal region (aa 364-1188) in pKT25This study
        pUT18-hptAhptA in pUT18This study
        pUT18C-hptAhptA in pUT18CThis study
        pKT25-hptAhptA in pKT25This study
        pKNT25-hptAhptA in pKNT25This study
        pUT18C-arctAarcA in pUT18CThis study
        pKT25-arcAarcA in pKT25This study
        pKNT25-arcAarcA in pKNT25This study
        pUT18C-SO_3457-172SO_3457 (barA) C-terminal region (aa 172-929) into pUT18CThis study
        pKT25-SO_3457-172SO_3457 (barA) C-terminal region (aa 172-929) into pKT25This study
  • a Apr, ampicillin resistance; Cmr, chloramphenicol resistance; Kmr, kanamycin resistance.