Phenotypic and genotypic characterization of denitrifying isolates

Genus (class or phylum) and isolateaIsolation conditionsDenitrificationcAerobic growthdSSU rRNA (rrs)Primer set (GenBank accession no.)eGenBank accession no. for draft genome sequence
Electron donorbNitrate concn (mM)N2O productionN2 productionnirKnosZnirKnosZ
Afipia (Alphaproteobacteria)
    3AS3*Acetate10NegPosPosFJ8514271 (GQ404513)6 (GQ404519)
    4AS1Acetate25NegPosFJ8514281 (GQ404514)6
    4LS4Lactate25FJ85143026 (GQ404520)
Hyphomicrobium (Alphaproteobacteria)
    1NES1*Ethanol0.5NegPosVWFJ8514384 (GU814013)6 (GQ404521)
    2NES1*Ethanol1NegPosFJ85143946 (GQ404522)
Rhodanobacter (Gammaproteobacteria)
    2AS1*Acetate1PosPosFJ8514433 (GQ404515)7 (GQ404523)
    2APBS1*APB1PosFJ8514443 (GQ404516)7 (GQ404524)GU233006, GU233007GU233008
Intrasporangium (Actinobacteria)
Bacillus (Firmicutes)
  • a An asterisk indicates that the isolate is presented in the phylogenetic tree (Fig. 1).

  • b Electron donor concentrations: acetate, 10 mM; lactate, 10 mM; ethanol, 20 mM; acetate, propionate, butyrate (APB), 10 mM.

  • c Production of nitrous oxide or nitrogen gas was tested by gas chromatography, as described in Materials and Methods. Neg, negative; Pos, positive; —, not tested.

  • d Pos, positive growth on agar medium under aerobic conditions; VW, very weak growth; —, not tested.

  • e Primer set number (Table 1) used for PCR amplification of the target gene. Amplification of 16S rRNA genes was performed using primers 27F and 1492R (Table 1). Neg, no amplification with any primer set for that functional gene.