TABLE 1.

Phaeoacremonium isolates used in this study

Phaeoacremonium speciesNo. of isolatesGenBank accession no.aOriginYr of isolation
P. parasiticum U31841 CBS860.73b,c,d1973
CBS113591e
2 (1)fPlant nursery in Murcia, Spain2004
2 (1)Plant nursery in Cataluña, Spain2005
1 (1)Cataluña, Spain2005
P. rubrigenum AF118139 CBS498.94c1994
P. inflatipes AF197990 CBS391.71c,d1966
CBS166.75e1974
P. mortoniae AF295328 CBS211.97e
CBS101585c,d1998
P. viticola AF118137 CBS113065e2001
CBS101738c,d1993
1 (1)Cataluña, Spain2005
P. angustius AF197974 g
CBS114992c,d1992
CBS114991e
P. krajdenii CBS109479d2001
P. venezuelense CBS651.85d1974
P. scolyti CBS113593d
CBS113597e1999
P. aleophilum AF017651 CBS246.91c,d1996
CBS110753e1998
1Toscana, Italy
16 (1)Ciudad Real, Spain2003
2 (2)Ciudad Real, Spain2004
4Cataluña, Spain2005
2Plant nursery in Portugal2004
5 (1)Plant nursery in Valencia, Spain2004
1Plant nursery in Murcia, Spain2004
2Plant nursery in Cataluña, Spain2005
  • a GenBank accession numbers of the ITS sequences used to design the primers and the discriminant restriction enzyme digestion patterns.

  • b CBS isolates are from the Centraalbureau voor Schimmelcultures, Baarn, The Netherlands.

  • c Ex-type strains used to optimize PCR conditions.

  • d Ex-type strains used to test PCR-RFLPs.

  • e CBS isolates used to validate the identification by PCR-RFLP.

  • f The numbers in parentheses are the numbers of field isolates identified by ITS sequences (and β-tubulin for P. viticola) used to validate the identification by PCR-RFLP.

  • g ITS sequence of CBS isolate 249.95. This was the original holotype of P. angustius, which was replaced with CBS114992 when lethally contaminated.