TABLE 1.

Effects of different factors on transcript levels of target internalin genes and opuCA, gadA, plcA, and prfA

GenebANOVA P values for the effect ofa:
StraincSalt stressdPresence of cellobiose or charcoale
opuCA<0.001<0.001<0.001
gadA<0.001<0.001<0.01
plcA<0.001<0.001<0.001
prfAf<0.001NS<0.01
inlA<0.001<0.01<0.001
inlB<0.001NS<0.001
inlC<0.05<0.001<0.001
inlC2<0.001<0.001<0.001
inlD<0.001<0.001<0.001
inlFNS<0.01<0.05
inlGNS<0.001<0.001
  • a NS, not significant.

  • b No analyses are reported for inlE, since inlE transcript levels were below qRT-PCR detection limits in all strains under all conditions.

  • c This factor comprises wild-type, ΔsigB, ΔprfA, and ΔsigB ΔprfA strains.

  • d This factor comprises both no exposure and exposure of cells to 0.3 M NaCl for 10 min.

  • e This factor comprises both the presence of 25 mM cellobiose and the presence of 0.2% charcoal in the growth media.

  • f Analysis for prfA was conducted only with data from 10403S and the ΔsigB strains. prfA transcript levels could not be determined for the ΔprfA and the ΔsigB ΔprfA strains, as the prfA primer and probe binding sites were deleted from these strains.