TABLE 2.

Relative cluster sizes and cluster centroids resulting from FCM clustering for ethanol-fixed samples hybridized with Bact0338a

SamplesClusterExpt 1Expt 2
Relative cluster size (%)Maximum intensityMean intensityValidatedRelative cluster sizeMaximum intensityMean intensityValidated
NF manure1 18.83 3.82 1.81 17.72 2.05 0.12
2 30.07 6.03 3.67 32.50 4.53 2.20
323.078.395.32+20.517.383.93+
414.3711.226.91+10.4211.475.82+
57.3015.269.36+6.8616.918.03+
63.5420.6012.10+4.6322.5410.36+
71.7827.9516.04+3.7630.4516.22+
80.6442.0919.84+2.5243.1118.81+
90.2767.7525.88+0.5472.1230.41+
100.12100.8226.75+0.5387.2752.78+
CF manure1 31.82 2.81 0.65 24.01 2.73 0.62
228.646.523.38+31.896.143.32+
317.5312.116.80+18.7310.515.82+
410.7919.7011.22+12.3016.688.45+
56.5229.3717.22+6.6425.7212.49+
62.1147.2424.45+3.2237.8117.08+
71.2171.2937.19+1.3457.2924.45+
80.79102.4240.76+0.8487.2939.82+
90.36123.9667.18+0.77143.0256.66+
100.23198.15155.97+0.27203.5690.83+
Soil1 9.87 2.31 0.30 10.93 1.64 −0.20
2 25.94 4.44 2.33 23.54 3.87 1.70
328.556.443.86+23.645.803.32+
419.278.785.54+19.137.994.69+
510.4411.977.89+12.8910.886.38+
63.6917.8311.17+5.2015.058.29+
70.7628.1413.02+2.7822.0010.80+
80.8636.5627.52+1.1530.7916.01+
90.3752.8838.96+0.5248.6325.42+
100.2687.2968.66+0.2397.5615.65+
  • a The values in bold type are below the threshold based on the average maximum intensity of cells in negative controls (no probe).