TABLE 3.

P. placenta genes encoding putative GHs with the highest transcript levels and/or positive MS/MS identification in BMA mediuma

Postia placentaPhanerochaete chrysosporium
ModelPutative functionAlleleSignal (log2) in:RatioP valueModelSignal (log2) in:RatioP value
GluBMAGluBMA
115648GH5 endo-1,4-β-glucanase*10896214.6215.041.330.0012 6458 12.1414.836.46<0.001
119525GH18 glycosidase12096014.8114.831.020.75601372379.009.711.640.0060
121831GH5 endo-β-1,4-mannosidase*13477213.4614.652.28<0.001 140501 9.0412.8914.44<0.001
112941GH16 endo-1,3(4)-β-glucanase*6180912.6614.583.79<0.0011083311.7713.653.680.0019
121713GH5 glucan 1,3-β-glucosidase4607712.7314.533.48<0.00112177410.1411.632.800.0092
117860GH72 1,3-β-glucanosyltransferase*11895014.2914.511.160.0206643313.8313.330.710.0232
134790GH27 α-galactosidase*9866212.7314.353.08<0.00113400111.6813.894.64<0.001
94557GH51 α-N-arabinofuranosidase*None13.0614.312.38<0.001 3651 12.2012.961.700.0017
127993GH35 β-galactosidase*12810113.6514.171.430.0033946611.2611.691.340.0709
116267GH55 glucan 1,3-β-glucosidase*10864813.3514.161.75<0.001 8072 12.0313.983.880.0832
100251GH51 α-N-arabinofuranosidase*12704612.3114.053.34<0.001365112.2012.961.700.00169
134924GH31 α-glucosidase*9387813.3313.511.130.031612546212.3812.621.180.373
113670GH10 endo-1,4-β-xylanase*13478712.6213.361.67<0.00113871511.1613.545.20<0.001
57564GH2 β-mannosidase*5657612.3213.352.05<0.00113538513.3713.571.150.118
126692GH79 glycosidase*11133211.7113.343.10<0.001199910.5511.882.520.0269
97540GH37 trehalase*11592913.1113.141.020.677014062713.9612.800.450.0254
112047GH92 α-1,2-mannosidase*11699213.1912.980.860.0134343112.7713.251.390.0109
126595GH18 chitinase*13492312.5912.861.210.16303987214.0213.710.810.172
112369GH20 β-hexosaminidase*6133112.9212.790.910.1450375229.059.081.020.781
117345GH15 glucoamylase*11311212.3412.661.250.042013881312.4313.021.510.0771
120395GH27 α-galactosidase*None11.5012.542.06<0.00112503312.2212.911.610.0193
115593GH47 Man(9)-α-mannosidase*13492513.4812.510.51<0.001455011.6213.443.52<0.001
110809GH43 galactan 1,3-β-galactosidase*None10.7512.503.38<0.00129710.6411.101.380.0625
116199GH5 glucan 1,3- β-glucosidase*12842512.1512.471.240.0066222012.4313.662.350.00274
111730GH28 polygalacturonase*4318910.5312.403.66<0.001 3805 10.9014.5712.70<0.001
54405GH16 endo-1,3(4)-β-glucanase*13502011.3012.191.86<0.001 123909 12.3313.271.930.0283
127469GH3 possible β-xylosidase*5121311.5812.081.420.0047 9257 9.2412.6710.79<0.001
107557GH3 β-glucosidase*None11.9512.021.060.500013906314.7014.550.900.599
46915GH3 β-glucosidase*9567711.0811.931.81<0.00112984911.2612.913.13<0.001
105534GH10 endo-1,4-β-xylanase*None8.3811.7110.06<0.0011383459.2310.432.290.014
55086GH31 α-glucosidase*1956410.9611.631.590.001496811.6412.732.120.0374
52194GH13 α-amylase*9288111.4511.621.130.13603835713.8613.600.830.141
62385GH92 α-1,2-mannosidase*4871611.3010.900.760.027813358512.0413.753.28<0.001
130398GH20 β-hexosaminidase*13490710.159.880.830.005214058713.2913.491.150.233
127046GH51 α-N-arabinofuranosidase*1002519.509.811.240.0053365112.2012.961.700.00169
95568GH5 endo-1,4-β-mannosidase*None8.919.521.52<0.001657912.4313.672.360.00279
  • a Calculations are essentially the same as described for Table 2. The genes or alleles are listed in order of decreasing transcript levels. Detailed scores, e-values, and alignment parameters for the best P. chrysosporium hits are listed in Table S1 of the supplemental material. *, detection of peptides by LC-MS/MS in BMA medium filtrate (see Tables S1 and S2). Underlined P. chrysosporium models correspond to genes with >50 cDNA tags in medium containing powdered oak (43). Data are ranked according to P. placenta log2 signals in BMA. The first eight genes exceeded the genome-wide average signal by 2 standard deviations (>14.24). Questionable GH51 models Ppl9457 and Ppl127046 lie within a 10-kb region of scaffold 110, and alignments suggest possible duplication or assembly error. The 3′ termini of P. placenta models GH20 Ppl130398 and GH10 Ppl113670 are problematic and require additional data and manual annotation. However, MS/MS protein assignments are based on reliable (5′) regions of these models.