TABLE 3.

Dispensable carbon sources for Vibrio cholerae metabolismd

Dispensable metaboliteParameter (gene presence)cCoefficientP valueNo. of strains per indicated cladee
ABCD
l-Aspartate612164
d-MannoseWater temp (−)0.4520.010161156
d-Serine610116
d-GlucuronateaTurbidity (+)−16.2370.016600104
d-Glucose-6-phosphate610126
α-Keto-glutarate69125
l-Glutamine610156
β-Methyl-d-glucoside51284
d-Fructose-6-phosphate610126
Glycyl-l-aspartate611164
Citric acidAmmonium (−)−2.9030.0438610164
l-Threoninea,b0000
d-Cellobiosea0100
l-Alanyl-glycine4691
p-HydroxyphenylacetateaSalinity (+)3.7230.01110016
TyramineaSalinity (+)3.7230.01110015
α-Cyclodextrina0200
Gelatina5695
Laminarina0200
N-Acetylgalactosaminea0610
Sialic acidMonth (−)−0.6740.02201830
  • a Dispensable substrates were not metabolized by the reference strain N16961; growth was observed for some isolates.

  • b No environmental isolates grew on l-threonine, but one clinical strain did.

  • c Log transformations were used to normalize salinity and ammonium data, and inverse transformations were used to normalize turbidity data. Polarity of association with gene presence is indicated by a positive (+) or negative (−) change in the parameter.

  • d Statistically significant (P < 0.05) correlations between dispensable substrate utilization and environmental parameters were verified by logistic regression and are displayed to the right of the dispensable metabolites to which they correspond.

  • e Number of strains in each clade which grew on each sole carbon source out of 6 strains for clade A, 13 strains for clade B, 16 strains for clade C, and 6 strains for clade D.