TABLE 1.

Media used for production of B. subtilis spores

Nutrient mediumNo. of isotopically distinct batches of water with which medium was prepared
Schaeffer's sporulation medium broth (31) made
    with three different nutrient broth powders4
Schaeffer's sporulation medium agar made with
    two different nutrient broth powders4
Schaeffer's sporulation medium broth with 0.2%
    glucose, made with two different nutrient
    broth powders4
Nutrient broth4
Tryptic soy broth4
Tryptic soy agar4
Luria broth4
Columbia agar4
2 g beef extract, 6 g peptone per liter1
4 g beef extract, 4 g peptone per liter1
6 g beef extract, 2 g peptone per liter1
2 g yeast extract, 6 g peptone per liter1
4 g yeast extract, 4 g peptone per liter1
6 g yeast extract, 2 g peptone per liter1
2 g beef extract, 6 g tryptone per liter1
4 g beef extract, 4 g tryptone per liter1
6 g beef extract, 2 g tryptone per liter1
2 g yeast extract, 6 g tryptone per liter1
4 g yeast extract, 4 g tryptone per liter1
6 g yeast extract, 2 g tryptone per liter1
2 g beef extract, 6 g peptone, 2 g glucose per liter1
4 g beef extract, 4 g peptone, 2 g glucose per liter1
6 g beef extract, 2 g peptone, 2 g glucose per liter1
2 g yeast extract, 6 g peptone, 2 g glucose per liter1
4 g yeast extract, 4 g peptone, 2 g glucose per liter1
6 g yeast extract, 2 g peptone, 2 g glucose per liter1
2 g beef extract, 6 g tryptone, 2 g glucose per liter1
4 g beef extract, 4 g tryptone, 2 g glucose per liter1
6 g beef extract, 2 g tryptone, 2 g glucose per liter1
2 g yeast extract, 6 g tryptone, 2 g glucose per liter1
4 g yeast extract, 4 g tryptone, 2 g glucose per liter1
6 g yeast extract, 2 g tryptone, 2 g glucose per liter1